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iseA [2017-10-15 16:44:17]
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iseA [2017-10-15 16:44:17]

inhibits in vitro activity of cell wall endopeptidases LytE and LytF, inhibits cell separation
locus
BSU18380
pI
10.18
mw
19.98 kDa
protein length
181 aa Sequence Blast
gene length
543 bp Sequence Blast
function
protection against cell envelope stress
product
inhibitor of autolysins
essential
no
synonyms
yoeB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.1|Autolysis]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    2,002,637 → 2,003,182

    The protein

    Structure

  • [PDB|2RSX] [Pubmed|23091053]

    • [SW|Localization]

  • cell membrane (according to Swiss-Prot), localized to cell separation sites

    • Expression and Regulation

    Operons

    genes
    [gene|0CA55371306D4BD768CFA82027DCB4D581BCCB87|iseA]
    description
    [Pubmed|22383849]

    regulatory mechanism

  • [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR]: repression, [Pubmed|17581128], in [regulon|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR regulon]

    • regulation

  • expression is modulated in response to D,L-endopeptidase activity (decreased upon reduced activity in the absence of [protein|4F9D70C4BB4FCA809BEAC341C3F8122FB7FD8872|CwlO] or [protein|8BF7EB539B53D75BD30BD30ABF27106D6A0B030B|FtsE]-[protein|E22D0F0AC56DC95AFC02E7F55FCD5FAA44B6B490|FtsX], increased upon overexpression of [protein|321F248C22D7283C0F3323F1F4069E36F8D7FE6C|LytE]) ([protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR]) [pubmed|31808740]
  • induced by vancomycin [Pubmed|17483219]
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    Biological materials

    Mutant

  • MGNA-B076 (yoeB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1075 NBRP B. subtilis, Japan]
  • BKE18380 (Δ[gene|0CA55371306D4BD768CFA82027DCB4D581BCCB87|iseA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATCGCTCTCTCCTTCA, downstream forward: _UP4_TAAGAAAAACGCCCGGTATA
  • BKK18380 (Δ[gene|0CA55371306D4BD768CFA82027DCB4D581BCCB87|iseA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATCGCTCTCTCCTTCA, downstream forward: _UP4_TAAGAAAAACGCCCGGTATA

    • References

  • 17581128,18761694,17483219,20059685,22383849,23091053,23033921,23199363